where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. Category 2 and 3 microplate readers use gradient heating as well. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. Make sure toPlate reader instructions Computer. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 96-well plates - Filter plates with 0. variety of. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. We would like to show you a description here but the site won’t allow us. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Top or bottom detection is available with a simple click of a button. Figure 2. The Versamax Microplate Reader is built around a grating monochromator. #E010) Pyroplate 96-well microplate (ACC cat. A. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 17,000 cells. Shaking: Linear, orbital, and double orbital. . 96-, 384-, and 1536-well microplates. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 7°C (gradient heating design). 000 to 2. Top or bottom detection is available with a simple click of a button. 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. Top and bottom reads are. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. Absorbance equation. Compare. MCF7 cells treated with. Molecular Devices L-Max II Luminescence Microplate Reader. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. 20 µL (96-well plates) or 10 µL (384. Description. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. In addition, users can define individual reagents and concentrations to be delivered to each well. QuickSync. Figure 1-2 Match chamfered corners of slide and adapter 2. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Optional 16-way manifold. These robust readers have been placed in labs from. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Fluorescence Microplate Reader Comparison Chart. Figure 3. g. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. 000 to 2. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. 190. #ABS Plus) Cell plating and treatment prior to assay. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. This study investigated the efficacy of fermented seaweed (Eucheuma cottonii) on the remediation of fluoride-contaminated soil. When a. Site. as well as all other Molecular Devices' microplate readers. ~ Bio-Tek ELx808 Microplate Reader $2,795. #ID5-STD) Pierce BCA Protein Assay Kit (ThermoFisher Scientific cat. absorbance microplate reader GEA. Primary considerations when choosing a microplate reader are throughput and flexibility. Two high-energy xenon flash lamps: Reading capabilities. Molecular Devices (UK) Ltd. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Can read 96 well plates (optional 384 well). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. $488. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Accurate, efficient nucleic acid quantitation is critical for molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. Packed 25 plates per sleeve, 2 sleeves per case. Versa Max. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. FlexIII. #iD5-STD). x. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. 96- and 384-well. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Pick the best speed, sensitivity, and price for your needs. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. 100 fmol 384-well (75 µL) 0. The. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Cell viability and cytoxicity assays. * Note: Programming the washer with a final aspirate height of 4. Values are averages from 5 clean plates, each filled with 200 µL water/well. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. FlexIII. CO 2 & O 2 control. Integrated stacker option. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. SpectraMax 250 User. x. Temperature control range from Ambient +4º to 50ºC. Our microplate. From the dropdown Menu, select Wellplates. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. No additional manuals are available. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. These lab workhorses are used in drug discovery. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Sort By: Show:. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Order Now. Highly selective for dsDNA over ssDNA or RNA. Sample by eye within . Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. Plus 384. 0200-6263. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Other. UV Plate 96 Well, Flat Bottom, Cs 50. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. They are also sealable using different types of adhesive mats foils or films. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. 003 fmol 0. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Part Number. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Endpoint, kinetic, spectrum. Uncontrolled Copy for Training and Reference Purposes Only . x. adjust the reader. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. On the day of the assay, the plate was equilibrated. , Corning, NY) or Greiner. PURPOSE. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. µl) was added to each well and incubated at 37°C for 1 h. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. EMax. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. The device was able to achieve 3σ limits of detection of ca. Wavelength(s):. 100 fmol 1536-well (8 µL) 0. $3,207. Microplate Readers. Microplate Readers include the M3, M4, M5, and M5e models. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. BMG Labtech - THERMOstar. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. #SpectraMax L Config) Methods. A barcode reader accessory can be purchased with theWe would like to show you a description here but the site won’t allow us. measurements in compatible Molecular Devices® microplate readers. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. This page contains the. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. 1% in white 96- and < 0. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. Contents of wells were pipetted up and down several times to mix. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. 5 µL of each sample was pipetted into a 96-well plate for PCR. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. An industry-renowned instrument for monitoring GPCRs and ion channels. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. 100 fmol luciferase per well. The 6-well plates were incubated at 37ºC for 9 days before picking. 1. Please refer to the Obsolete Microplate Reader Models for more information. Absorbance cuvette port. Visit the supplier page or submit a quote to learn more. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. Please click on the links below to view the Microplate Reader Comparison Tables. 25, 3. This is a Molecular Devices Thermomax 96 well Absorbance Microplate Readers. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. • 0. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. The system can perform readings. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. Molecular Devices has provided scientists with . Supersedes 1. Turn the instrument around so that the back of the instrument is. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 1 fmol luciferase per well. Learn how you can utilize microplate readers to drive landmark discoveries. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 1 OD at 230 nm. D. Fluorescence Microplate Reader Comparison Chart. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Temperature control: 4°C above ambient to 45°C. p-Nitroaniline - Catalog No. 5°C at 37°C. 1. This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. C. Click Create. x. $542. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. with Quartz ABS Plastic. 00 . At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. Lucid Scientific. Align the two slots on the bottle holder with the two pegs on the rail. White 96-well platePlate formats. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. e 3370 Costar 96 Well. Back plate and…. 0 . 0 OD. minutes and begin reading microplates with seven mouse clicks. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. REF (SpectraMax. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. Each wellThe Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The CellTiter-Glo assay was used to measure ATP. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. x. #23225). SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. T = I/I 0 and %T = 100 (T). The color touch screen provides a visual user interface, making programming fast and intuitive. 96-, 384-, and 1536-well microplates. S. x. . 76358-624. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. An additional reference channel for optimizing the light intensity; Auto switch-off of the light source when not in use to extend lifetime; An automated self-check of major components at instrument start-upThe EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. one 96-well sample plate can be controlled from 15–40°C,. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. VMax . No accessories are needed and SoftMax® Pro Software. Figure 1. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. #CA961) Methods. This reader can also be used as a standard. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Packed 25 plates per…. High tolerance for contaminants. 2. 4 (Molecular Devices cat. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 012 fmol 0. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…AquaMax® Microplate Washers are configurable with user interchangeable heads for both 96- and 384-well microplate washing applications, including plate or cell washing. 500 USD. #CA961) Methods. Temperature variation ± 0. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. For biochemical assays, the washer removes unbound material and unreacted reagents. 00 USD. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. 1 mL/well of media. Deep well blocks available in 24, 96 and 384-well formats An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. High-throughput, automated dynamic light scattering. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. These models can no longer be repaired. Figure 2-23: Bottle holder To install the bottle holder: 1. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. All trademarks used herein are the property of. Microplate format. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. #ABS PLUS) Methods. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hourMicroplate Reader, For Lab. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Plus 384. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. It also integrates with the. Published Dec 16, 2020 | Updated Nov 07, 2022. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. A control well was set up to verify. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. The drawing shows a standard microplate of 15 mm height covered with a lid. Molecular Devices SpectraMax Plus 384 Absorbance. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Microplate readers, SpectraMax®, with SpectraDrop. 0% ± 0. 3) At the bottom of the strip, click on "Show. 00. a white 96-well half-area microplate (Corning cat. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Our. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. U-bottom: 30-40 µl. These robust readers have been placed in labs from Antarctica to the. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Optimized for fluorescence microplate readers. For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. #C1500) Control standard endotoxin (ACC cat. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Controlled by the computer. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. Features. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Ensure that cells reach around 80–90% of confluence on the day of the. These microplates have a corner notch for use in standard plate readers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 2 with 1000 cells. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 005 OD Normal and Rapid modes, 96- and 384-well plates;. Published Dec 16, 2020 | Updated Dec 16, 2020. x. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. the use of a reader calibrated with primary NIST and NMI standards. Reader Documentation Version No. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Answer. We would like to show you a description here but the site won’t allow us. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,.